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The Protective Role of Green Tae and Ginkgo biloba Extract against Aging Dysfunction Induced by D-Galactose in Rats

Shwan H.Sofy, Esmail S.Kakey & Sarab D.alshamaa

The current study was aimed to investigate the potential anti-aging role of Green Tae and Ginkgo biloba extract in hepatic, cardiac and renal aging dysfunctions induced by D-galactose in rats. Aging was induced by daily intraperitoneal injection of D-galactose (300 mg/kg dissolved in 1 ml DW) for 9 weeks to accelerate senescence and aging induction. The rats were randomly divided into four groups (7 rats per group). The rats of the first group (G I), were kept on normal feeding without any treatment and regarded as non aged control group, the rats of the second group(G II),were injected by D-galactose daily for 9 weeks, and regarded as induced aged group, the rats of the third group (GIII) , were injected daily with D-galactose(300 mg/kg ) and, orally treated with green tea extract (200 mg/kg) daily for 9 weeks , the rats of the fourth group (G IV) were daily injected by D-galactose (300 mg/kg) and orally treated with ginkgo biloba extract (200 mg/kg) for 9 weeks. The results showed significant increase(p<0.05) in cardiac and hepatic enzymes levels for serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) , Alanine phosphatase (ALP),α - Glutamyle transferase (GGT), Lactate dehyderogenase (LDH) and Cretinine phosphokinase(CPK) in D-galactose induced aged group. The treatment of the D-galactose inducing aged rats with green tea extract(200 mg/kg) and ginkgo biloba extract (200 mg/kg), appeared a protective role against aging dysfunctions induced by D-galactose , and caused significant (p<0.05) decrease in the levels of hepatic and cardiac biochemical markers, in the serum levels of AST, ALT, ALP, GGT, LDH and CPK levels as compared with D-galactose induced aged rats. With respect to the renal function test parameters, D. galactose (300 mg/kg body weight) injection for 9 weeks caused elevation in the levels of urea and creatinine but a decrease in uric acid, albumin and total bilirubin as compared to non treated control group rats. Green tea extract (200 mg/kg) and Ginkgo biloba extract (200 mg/kg) treatments of Dgalactose injected rats, showed increase in levels of uric acid, albumin and total bilirubin with non significant decrease in the urea and creatinine levels as compared with the D-galactose induced aging control rats. In conclusion the results of the current investigation revealed the protective role of Green tea extract and Ginkgo biloba extracts, in suppression of senescence markers and aging dysfunction in hepatic, cardiac and renal functions, contributes to oxidative stress and apoptosis induced by D-galactose in rats.