Журнал интеллектуального анализа данных в геномике и протеомике

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The Complete Mitochondrial Genome Sequence Variation and Phylogenetic Analysis of Mulberry

Guo Liangliang, Shi Yisu, Wu Mengmeng, Michael Ackah, Qiang Lin, Weiguo Zhao*

Background: Mulberry is an economically significant crop, tolerance to various environmental conditions. The plant (leaves) is use for feeding silkworm and, for its landscaping and possesses high development prospects and scientific research value. Mitochondria are the plants' powerhouse that produces the required energy to carry out life processes.

Objective: Plant mitochondria (mt) genome serves as a powerhouse that produces the required energy to carry out life processes in plants. However, the mitochondria (mt) genome of mulberry plant is still unexplored. This study investigated the mt genome of Morus L (M. atropurpurea and M. multicaulis) and compared it to other plant species.

Methods: The mt genome of Morus L (M. atropurpurea and M. multicaulis) were sequenced using Oxford Nanopore Prometh ION and data assembled and analyzed and then compared to other plants mitochondrion genome. Phylogenetic analysis was arried on to study the evolution status of the mulberry plants studied

Results: The circular mt genome of M. multicaulis has a length of 361,546 bp, contains 54 genes, including 31 protein-coding genes, 20 tRNA genes, and 3 rRNA genes and composition of A (27.38%), T (27.20%), C (22.63%) and G (22.79%). On the hand, the circular mt genome of M. atropurpurea has a length of 395,412 bp long, comprises C+G (45.50%), including 57 functional genes containing 2 rRNA genes, 22 tRNA genes and 32 PCGs. There exist sequence repeats, RNA editing gene and migration from cp to mt in the M. multicaulis and M. atropurpurea mt genome.

Phylogenetic analysis based on the complete mt genomes of Morus and other 28 species reflect an exact evolutionary and taxonomic status.

Conclusion: We found out that the Morus species mt genome is circular, with M. multicaulis having a length of 361,546 bp. 54 genes, including 31 protein-coding genes, 20 tRNA genes, and 3 rRNA genes. Also, M. atropurpurea was found to have a length of 395,412 bp. Moreover, a total of 57 genes contains 32 protein-coding genes, 22 tRNA and 3 rRNA were annotated in the genome. The results will provide a comprehensive understanding of the Morus mt genome and may help in future studies and breeding of mulberry varieties.