Журнал наномедицины и нанотехнологий

Абстрактный

Study on Cell Viability, Skin Penetration, and Edoema Inhibition Using Collagen-based Silver Nanoparticles

Saura Silva

The goal of this study was to examine the behaviour of silver nanoparticles stabilised with collagen (AgNPcol) and to assess the skin's ability to absorb the substance and how it responded to carrageenan-induced paw edoema. Utilizing solutions of the reducing agent sodium borohydride (NaBH4), silver nitrate (AgNO3), and collagen, AgNPcol was created. The characterization process used dynamic. With 30% of all proteins and making up 6% of the human body's weight, collagen is thought to be the most plentiful protein in the animal kingdom. In order to comprehend the stability of the nanoparticle system, the impacts seen in biological systems and the emergence of new chemical medicinal products, studies that look at the interaction between silver nanoparticles and proteins have been highlighted in the literature. AFM, X-ray diffraction, and dynamic light scattering (DLS) were used for characterization. In order to examine the cellular viability of human melanoma cancer (MV3) and murine fibroblast (L929) cells, flow cytometry was used. Using a Franz diffusion cell, the skin permeation investigation was carried out, and AgNPcol's effectiveness in preventing mouse paw edoema was assessed. AgNPcol has a hydrodynamic diameter of 140.7 7.8 nm and a zeta potential of 20.1 0.7 mV, respectively. AgNPcol failed to cause necrosis, late apoptosis, or early apoptosis in L929 cells, although it was more hazardous to cancer cells (MV3) than to healthy cells (L929). AgNPcol promoted skin penetration and avoided paw edoema while showing elevated toxicological effects in cancer MV3 cells.