Фармацевтика Аналитика Акта

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Simultaneous RP HPLC Determination of Camylofin Dihydrochloride and Diclofenac Potassium in Pharmaceutical Preparations

M.V. Rathnam and R. R. Singh

A simple, fast and precise reversed phase high performance liquid chromatographic method has been developed for the simultaneous determination of Camylofin dihydrochloride and Diclofenac Potassium using Methylparaben as an internal standard. Efficient __ chromatographic separation was achieved on Inertsil C 18 column (250mm x 4.6 mm, 5μm) as stationary phase with a mobile phase comprising of 0.05 M KH 2 PO 4 in water : Methanol (35:65,v/v) at a flow rate of 1.5mL min -1 , column temperature of 27°C and UV detection at 220 nm. The retention time of Methylparaben, Camylofin dihydrochloride and Diclofenac potassium were 3.60 min, 4.85 min and 13.10 min respectively. The proposed method was validated for linearity, accuracy, precision, sensitivity, robustness and solution stability. Linearity, accuracy and precision were found to be acceptable over the ranges of 250-750μg mL -1 for both camylofin dihydrochloride and diclofenac potassium. The test solution was found to be stable for 48 h. It can be conveniently adopted for routine quality control analysis.