Журнал бактериологии и паразитологии

Абстрактный

Prevalence of inhibitor-resistant beta-lactamase-producing Escherichia coli in humans and poultry in Bangladesh

Mr. Anowar Khasru Parvez, Mahfuza Marzan, Syeda Moriam Lisa, Tasleen Jahan Mou, Ishrat Jahan Azmi, Mr. Shahedur Rahman and Zahid Hayat Mahmood

Human clinical specimens (n=48) and poultry faecal specimens (n=40) were collected from a sub-district (Sawar) of Bangladesh. A total of 25 E. coli were isolated from these specimens . TheseE. coli were tested for antibiotic susceptibility against commonly used antibiotics. In addition, double synergy test (DDS) and PCR method were used to detect ESBL (extended spectrum beta-lactamase) producers. Twenty-three E. coli were multiresistant, i.e. resistant to at least three different groups of antibiotics. DDS method showed that all E. coli from poultry samples and 78% of E. coli from clinical samples were resistant to amoxicillin and clavulanic acid combination. Thus, they were phenotypically confirmed as inhibitor-resistant beta-lactamase producers. PCR result showed only blaTEM gene in E. coli from poultry samples. Thus, inhibitor-resistant type of β-lactamase was found to be dominant in E. coli from both types of samples.