Журнал бактериологии и паразитологии

Абстрактный

Molecular study of Theileria annulata in cattle by PCR method - RFLP method in Iran

Jamal Akbari, Javad Javanbakht, Moussa Tawassouli, Mohammad Tabatabai and Rahmer Shafii

Theileriosis is one of the infectious diseases of cattle and buffalo in tropical countries and Mediterranean region which has significant economic value, caused by Theileria haemoprotozoan and transmitted by veterinarians belonging to Ixodidea. The present study investigates 52 samples in western and eastern Azerbaijan, Sanandaj and Kermanshah in Iran (2009). In this study, Theileria annulata was separated from diseased and healthy cattle by PCR-RFLP method. For this procedure, special primers were obtained from SmI-2 gene. 270 bp fragment was separated from the collected blood samples. PCR products were isolated by agarose gel and ultraviolet ray system. Among 22 naturally infected animals, 13 cases (59.09%) were positive in blood smear and 18 (81.81%) were positive in PCR. Among randomly selected healthy cattle animals from 30 cases, 4 cases (13.32%) were positive in blood smear and 5 (61.66%) were positive in PCR. In order to investigate the genetic variability (PCR-RFLP), the isolated 270 bp fragment was digested with restriction enzymes such as TaqI, BSURT, AIUI, RsaI. TaqI enzyme generated fragments (200, 170 bp), AIUI enzyme generated fragments (120, 90, 60 bp) and (150, 90, 30 bp), and RsaI and BSURI enzymes could not digest the considered fragment. Finally, six different patterns of genetic variability of the corresponding gene were found.