Журнал патологии растений и микробиологии

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Molecular Detection and Identification of Begomovirus Isolate on Tomato from Central Region of India

Sunil Kumar Snehi, Shilendra Singh Parihar, Govind Gupta, Vinod Singh and Anita Singh Purvia

The natural occurrence of leaf curl and blisters disease on tomato was observed at Bhopal, India in the January, 2015. The begomovirus was amplified on symptomatic tomato by polymerase chain reaction (PCR) using coat protein gene specific primers. The purified PCR product (~800 bp) was sequenced and submitted in GenBank database (KU760803) and identified by their sequence analyses. The isolate under study (KU760803) showed 97% to 99% sequence identities and closest phylogenetic relationships with various isolates of Tomato leaf curl New Delhi virus (ToLCNDV), therefore, the isolates under study were identified as isolates of ToLCNDV associated with leaf curl and blister disease on tomato in the first time from central region of India.