Журнал бактериологии и паразитологии

Абстрактный

Molecular characterization of enterotoxigenic Escherichia coli : effects on intestinal nitric oxide in diarrheal diseases

Muhammad Arfat Yamin1, Ebuka Elijah David2*, Humphrey Chukwuemeka Nzelibe3, Muhammad Nasir Shuaibu3, Rabiu Abdussalam Magaji4, Amakaeze Jude Odugu5 and Ogamdi Sunday Onwe6

The aim of this study was to investigate the effect of enterotoxigenic E. coli (ETEC) diarrhea on the expression of fecal nitric oxide (NO) and intestinal inducible nitric oxide synthase (iNOS) in rats. E. coli isolates were obtained from infant diarrhea samples. Multiplex polymerase chain reaction (PCR) was used to detect the presence of the eltA gene for heat-stable (ST) enterotoxigenic E. coli and eltB for heat-labile (LT) enterotoxigenic E. coli . Disk diffusion was used to determine the susceptibility of organisms to antibiotics. Biofilm formation was determined using thiazoyl blue tetrazolium bromide dye in a 96-well plate. Fecal NO was measured using a standard Griess reaction system. Reverse transcription PCR was used to investigate the expression of iNOS. Although none of the ETEC isolated in this study belonged to the classical serotype, serogroups O6 and O8 were found to be associated with ETEC. Among the three ETEC, two were found to be multidrug resistant. The biofilm formation ability of all ETEC was found to be between weak and moderate biofilm producers. Faecal NO was found to be elevated in both LT and ST diarrhea groups, but there was no corresponding expression of intestinal iNOS. This suggests that the elevated NO may have resulted from upregulation of constitutive NOS rather than iNOS.