Журнал бактериологии и паразитологии

Абстрактный

Modified microbial adhesion assay to colony-forming unit hydrocarbons and assessed cell surface hydrophobicity and biofilm formation of Vibrio scophthalmi

Guo Qiao, Hua Li, De-Hai Xu, and Su Il Pak *

Vibrio scophthalmi is considered an opportunistic pathogen of flounder. Little information is available on the adhesion of V. scophthalmi to the host, which is an important step in the initial infection process. The objectives of this study were to (1) develop a modified microbial adhesion to hydrocarbon (MATH) assay using the colony forming unit (CFU) method and evaluate the cell surface hydrophobicity of V. scophthalmi, (2) detect the mucus layer and biofilm production of V. scophthalmi, and (3) investigate the motility and antibiotic susceptibility associated with biofilm production of V. scophthalmi. The results showed that the cell surface hydrophobicity of V. scophthalmi was moderate as determined by the salt aggregation test (SAT) and MATH assay. The fraction distributed in the hydrocarbon phase (%Adh) was significantly higher with the modified CFU MATH assay than with the classical MATH assay on a spectrophotometer and MATH assay on a microscope. V. scophthalmi produced a slime layer and biofilm. Optimal conditions for biofilm production for V. scophthalmi were BHIB or TSB medium supplemented with 2% NaCl and 0-0.5% glucose and incubated for 24 h. The level of biofilm production is associated with the pathogenicity of V. scophthalmi and susceptibility to antibiotics. V. scophthalmi was motile with flagella-mediated swimming and type IV pili-mediated twitching, but without swarming.