Фармацевтика Аналитика Акта

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Micellar Liquid Chromatographic Determination of Lamivudine, Indinavir and Ketoconazole in Dosage Forms and Biological Fluids

Wael Talaat

A simple, reversed phase high performance liquid chromatographic method has been developed for the determination of lamivudine, indinavir and ketokonazole in pharmaceutical preparations, human plasma and urine. The method was conducted using A Shim-pack VP-ODS (150×4.6 mm i.d) stainless steel column at ambient temperature with ultra violet detection at 225 nm. Micellar mobile phase consisted of 0.07 M sodium dodecyl sulphate , 10% n-propanol, 0.3% triethylamine in 0.02 M phosphoric acid (pH 4.5) was used and pumped at a flow rate of 1.2 mL/ min . The calibration curve was rectilinear over the concentration range of (0.05-1.0) μg/mL and (0.2-5.0) and (0.3-5.0) μg/mL lamivudine , indinavir and ketokonazole respectively. The proposed method was successfully applied to the analysis of these drugs in some dosage forms.