Журнал интеллектуального анализа данных в геномике и протеомике

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In Silico Functional Prediction, and Expression Analysis in Response to Drought Stress of Natural Resistance-Associated Macrophage Protein (NRAMP) Gene Family in Maize

Md. Golam Rabby, Md. Mostafa Kamal, Md. Sakib Hasan, Md. Mossabbir Hossain, Md. Numan Islam*

Natural Resistance-Associated Macrophage Proteins (NRAMPs) function in plants as metal transition transporters to maintain metal homeostasis. The genome-wide identification and functional prediction of the maize NRAMPs have not yet been studied. The genome-wide analysis identified seven (ZmNRAMP1, ZmNRAMP2, ZmNRAMP3, ZmNRAMP4, ZmNRAMP5, ZmNRAMP6, ZmNRAMP7) proteins in maize with a molecular weight of 29.08 (ZmNRAMP2) to 63.20 (ZmNRAMP5) kDa. Except for ZmNRAMP1 and ZmNRAMP3, all ZmNRAMPs are essential proteins (isoelectric points greater than 7). The number of introns in ZmNRAMPs ranges from 1 to 13, which is almost consistent among the phylogenetic groups (A and B) between maize and Arabidopsis. All proteins showed multiple motifs (ranging from 6 to 10) that were consistent among the groups between maize and Arabidopsis. We also conducted transmembrane domains, cis-regulatory elements, chromosomal mapping, and gene ontology annotation that revealed significant functional attributes. Significantly increased ZmNRAMPs (ZmNRAMP1, ZmNRAMP2, and ZmNRAMP4) expression in vegetative and reproductive tissues may play an essential role in heavy metal absorption, transport, and homeostasis. During drought stress, significant upregulation of cc (ZmNRAMP1, ZmNRAMP2, ZmNRAMP4, and ZmNRAMP7) may play a role in drought tolerance or acclimatization in maize.