Журнал бактериологии и паразитологии

Абстрактный

Идентификация методом секвенирования гена рибосомальной РНК 16S молочнокислых бактерий, продуцирующих антибактериальные вещества из плодов лангсата (Lansium domesticum) в округе Северная Минахаса, Северный Сулавеси

Хелен Джоан Лавалата*, Мариана Ренгкуан, Утари Сатиман

Fourty two strains Lactic Acid Bacteria (LAB) were isolated from Langsat fruit (Lansium domesticum) samples obtained from 4 districts in Minahasa, North Sulawesi. 12 isolates of lactic acid bacteria were isolated from Langsat fruit in North Minahasa District. Lactic acid bacteria have the ability to inhibit the growth of pathogenic bacteria and putrefactive bacteria. The aim of this study was to identify LAB isolates producing antibacterial agents from Langsat fruit at North Minahasa District based on identification genotypic. Identification genotypic isolat LMU7 from Langsat fruit was based on 16S rRNA gene sequencing Method. One Isolate (LMU7) had a great ability to inhibit the growth of pathogenic bacteria and putrefactive bacteria. The 16S ribosomal RNA gene from one isolate could be amplified by PCR (Polymerase Chain Reaction) and show a single band on a 2% (w/v) agarose gel. Identification by 16S Ribosomal RNA gene showed that one isolate LAB producing antibacterial agents were identified as Lactobacillus plantarum with a similarity index of approximately 99.93%.