Фармацевтика Аналитика Акта

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Development of Celecoxib Complexes: Characterization and Cytotoxicity Studies in MCF-7

Md Aftab Alam, Mohd Aamir Mirza, Sushama Talegaonkar, Amulya K Panda and Zeenat Iqbal

The objective of the study was to compare the complexing ability of Celecoxib with different complexing agents. Inclusion complex of Celecoxib is well established in literature but its evaluation in terms of thermodynamics was not studied elaborately. Hence after characterization of complexes with Differential scanning calorimeter, X-ray diffraction, NMR and % complexation efficiency, its solvation energetics and thermodynamics were determined. Complexation ability of celecoxib with caffeine was also evaluated, where non-inclusion (charge transfer) mechanism is involved. So, these two mechanisms of complexation were also tested with respect to each other. In this manuscript, new complexing agents (humic acid and fulvic acid) were also introduced for Celecoxib. These lie in the category of natural organic matter and we explored an indigenous source (Shilajit) to extract it. Other agents used for the development of complex were HP-β-CD and β-CD. Release mechanism of drug from complexes was studied by in vitro release studies. MTT assays were also performed to assess in vitro cell toxicity potential of the complexes in comparision to neat drug.