Журнал бактериологии и паразитологии

Абстрактный

Detection of Salmonella in Food Samples by Culture and Polymerase Chain Reaction Methods

Omar B. Ahmed, Atif H. Asghar, Ibrahim H.A., Abd El-Rahim and Hegazi A.I.

Traditional culture methods for isolation and identification of foodborne bacterial pathogens are quite sensitive and inexpensive, but at the same time, they are labor-intensive and time-consuming. Molecular methods are more rapid and highly sensitive for identification of foodborne pathogens. This study was conducted to evaluate a 12-hour PCR method for the detection of Salmonella in food samples. The results showed that out of 150 food samples, 32 (21.3%) were culture positive, 35 (23.3%) were PCR positive, the sensitivity of PCR was 100% and the specificity was 97.5%. The study concluded that 6-hour enrichment followed by PCR was a rapid, simpler method that could detect Salmonella within a maximum of 12 hours.