Журнал исследований и терапии стволовых клеток

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Control of Simultaneous Osteogenic and Adipogenic Differentiation of Mesenchymal Stem Cells

Rong Cai, Tomoko Nakamoto, Takashi Hoshiba, Naoki Kawazoe and Guoping Chen

Mesenchymal stem cells (MSCs) are pluripotent cells which can differentiate into several distinct lineages such as osteoblasts and adipocytes. Osteogenesis and adipogenesis of MSCs induced with specific induction factors have been well exploited. However, it is not clear how simultaneous osteogenesis and adipogenesis of MSCs is balanced by the composition of induction medium. In this study, MSCs were cultured in the mixture medium at different ratio of osteogenic medium (OM) and adipogenic medium (AM) to investigate the simultaneous osteogenesis and adipogenesis of MSCs. Differentiation was investigated by histological staining and real-time PCR analysis. The results showed that adipogenesis was dosage dependent on the concentration of adipogenic induction factors, while osteogenesis was not. The balance of osteogenesis and adipogenesis could be control by different ratio of osteogenic and adipogenic media. The results will be useful for controlling stem cell differentiation.