Журнал бактериологии и паразитологии

Абстрактный

Comparison of multidrug-resistant Acinetobacter baumannii isolates from French and Tuscan hospitals

Ben Othman A, Bouroukoa S, Battih H, Zribi M, Masmoudi A and Fendry S

The aim of this study was to evaluate whether there are differences between the clustering of isolates collected between 2003 and 2005 from two different patients in Tunisia and France. Methods: A sample of 62 A. baumannii isolates was studied; 31 from a French hospital (Poitiers) and 31 from a Tunisian hospital (Rabta). Antibiograms were obtained using the disk diffusion method. The presence of class 1 and 2 integrons was studied by PCR. Molecular relationships were studied by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE). Sequence typing of the adeB gene was determined to identify intraspecific groups. Results: The present study successfully focuses on the results of the epidemiological process between the two patient studies. PFGE and RAPD methods were useful to distinguish epidemic and endemic clones in the two study collections. Sequence analysis of an 850-bp internal fragment of the adeB efflux gene revealed 9 new sequence types (STs). Conclusion: We did not detect significant changes related to the epidemiological situation. This study showed that different genetic types of A.baumannii were detected in the collection of two strains. Epidemic diseases were mainly limited to the intensive care unit and persisted for the two years of the study. However, more control procedures are used for these clones in the Tunisian hospital.