Журнал канцерогенеза и мутагенеза

Абстрактный

Antitumor Activity of Atractylenolide II on Breast Cancer Cells through Regulation of Estrogen Receptor Protein Expression and NF-Kb Signaling Pathways

Chao Yang, MI Nasser, Shuoji Zhu, Chen Chen, Salah Adlat, Muqaddas Masood, Zhu Ping and Nan Jiang

Many women in the world are suffering from breast cancer deeply. Although many efforts are made in the study of breast cancer prevention and treatment, little attention is paid to the molecular mechanism of the disease. In the present study, considerable techniques such as western blotting, quantitative RT-PCR (qRT-PCR), Luciferase Immunohistochemistry, and flow cytometry analysis has been performed to analyze the effect of Atractylenolide II(ATR II) addiction for breast cancer research. As indicated by our research outcomes, ATR II could prohibit the proliferation of Prostate cancer as well as Breast cancer cells, in particular, ATR II induces MDA-MB231 and MCF-7 cells apoptosis, through G2/M -phase cell arrest. Also, cell apoptosis was induced by ATR II mainly associated with extrinsic mitochondrial pathways followed by activation of death receptor (DR4) that regulated activation of caspase-8 through cascade promotes activation of caspase-3, and therefore, drive breast cancer cells line to apoptosis. The apoptosis induced by ATR II is also associated with its ability to regulate the activity of androgens receptors and inhibition of NF-κB signaling pathways. Regarding this finding, ATR II might be promising chemotherapies drugs for breast cancer cells lines.