Lourdes Reyes-Escojido, Ingrid Rodriguez-Buenfil, Jesus Valdez, Luis Kameyama, Odila Saucedo Cardenas and Francisco Martinez-Perez
Here we report a universal metagenome purification method for identification of uncultured species from sediments and soils using nested PCR-DGGE according to 16S rDNA. This combination of methods uses in situ enzymatic lysis, polyvinylpyrrolidone (PVP), Chelex 100, glass beads-silica gel and chaotropic salts, with the advantage that it can be applied to different soils.